Microbial Genetics

Course: M.Sc Code: MB-103 Branch: Microbiology Year: 2023

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Roll No. ................................
Total No. of Questions : 11
[Total No. of Printed Pages : 8

M.Sc. 1st Semester (REG/ATKT)

Examination, 2023-24

Microbiology

Paper - MB-103

Microbial Genetics

Time : 3 Hours]
[Maximum Marks : 85
Note :- Attempt all questions.
SECTION - 'A'
Objective Type Questions
1×15=15
1.
Choose the correct answer:
(i) The gene transfers in microorganisms occur via the transposons or 'jumping genes' these are also a.............
  1. RNA molecule
  2. Genome
  3. Plasmids
  4. DNA molecule
(ii) Which of the following enzyme unwind DNA:
  1. DNA helicase
  2. DNA polymerases
  3. Nucleases
  4. None of the above
(iii) Which of the following refers to the mechanism of horizontal gene transfer naturally responsible for the spread of antibiotic resistance genes within a bacterial population:
  1. Conjugation
  2. Transformation
  3. Specialized transduction
  4. Generalized transduction
(iv) During elongation step in translation, to which ribosomal site does an incoming charged tRNA molecule bind:
  1. A site
  2. P site
  3. E site
  4. B site
(v) The Adenine - Thymine base pair is held together by:
  1. 3
  2. 5
  3. 2
  4. None of the above
(vi) Klenow Fragment of DNA polymerase I lacks:
  1. 3' to 5' exonuclease activity
  2. 5' to 3' polymerase activity
  3. 5' to 3' exonuclease activity
  4. None of the above
(vii) Which of the following nitrogenous bases are absent in RNA:
  1. T
  2. A
  3. G
  4. C
(viii) Which is the start codon:
  1. UAA
  2. AUG
  3. UAG
  4. UAC
(ix) Which of the following antibiotics target protein synthesis:
  1. Penicillin
  2. Bacitracin
  3. Chloramphenicol
  4. None of the above
(x) What is DNA replication:
  1. Conservative
  2. Semi-conservative
  3. Non-conservative
  4. All of the above
(xi) Which of the following enzymes is the principal replication enzyme in E. coli:
  1. DNA polymerase I
  2. DNA polymerase II
  3. DNA polymerase III
  4. None of the above
(xii) Which enzyme used to join bits of DNA:
  1. DNA helicase
  2. DNA polymerases
  3. Nucleases
  4. DNA ligase
(xiii) How many structural genes are present in a lac operon:
  1. 2
  2. 5
  3. 3
  4. 4
(xiv) Which of the following is responsible for the switching on and off of the lac operon:
  1. Fructose
  2. Maltose
  3. Lactose
  4. Ethanol
(xv) Introduction of DNA molecules into the recipient organism is termed as...............
  1. Transformation
  2. Transcription
  3. Translation
  4. None of the above
SECTION - 'B'
Short Answer Type Questions
5×5=25
2.
Describe basic features of genetic code.
OR
What is capping and polyadenylation of mRNA.
3.
Explain the role of various factors in tremination of protein synthesis.
OR
Describe inhibitors of protein synthesis.
4.
What do you mean by nucleotide excision repair?
OR
What do you mean by SOS repair? Explain.
5.
What is the mechanism of action of topoisomerase?
OR
What are SSB? What is its role in DNA replication?
6.
What are the various modes of replication? Explain briefly.
OR
What do you mean by base excision repair?
SECTION - 'C'
Short Answer Type Questions
5×9=45
7.
Describe lac operon.
OR
Explain tryptophan operon.
8.
Describe transcription process in details.
OR
Explain different types of RNA polymerases.
9.
Describe transformation.
OR
Describe transduction.
10.
Explain continuous and discontinuous synthesis in replication.
OR
What are the differences between prokaryotic and eukaryotic genetic material. Explain with diagram.
11.
What are the inhibitors of RNA synthesis. Explain.
OR
Describe structural features of tRNA.